oligonucleotide probe meaning in English
低核苷酸探测剂
Examples
- Calculate the distibution of the melting temperature of the oligonucleotide probe sets that affymetrix uses for its microarrays
计算用于微阵列的基因表达谱、和基因分型研究技术平台使用的、低聚核苷酸探针装置的溶解温度分布。 - 2 . the optimize of the oligonucleotide probes preparation the optimal condition is to resuspend the probes in ph = 9 , 0 . 1 m carbonate buffer to 100 m . 3
2 .寡核昔酸探针制备条件的优化以ph二9 ， 0 . im的碳酸盐缓冲液，重悬为100林m探针为最优条件。 - Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end . then resuspended the lyophilized probes in ph = 9 , 0
寡核苷酸探针的制备根据genbank数据库新近公布的hla - dqa1等位基因序列，设计四组共16条特异性寡核苷酸分型探针，并在各5 ’端做氨基修饰。 - In the present experiment studies , an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ) , cyclooxygenase - 2 ( cox - 2 ) , glial cell line - derived neurotrophic factor ( gdnf ) , caspase - 3 mrna and protein after brain injury in rats . immunocytochemical staining , western blotting , nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna , cox - 2 mrna , gdnf mrna , and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )
本实验从自行设计大鼠脑损伤落体打击器开始，先行建立了一个便于观察和施加处理因素、控制性好、重复性好的动物模型，选用30g击锤从25cm高处下落，冲击应力d为355 . 09kpa ，打击大鼠右顶部，造成中等程度的闭合性脑损伤，从病理形态学、组织超微结构观察及微管相关蛋白- 2 ( microtubuleassociatedprotein2 ， map - 2 ) 、环氧合酶- 2 ( cyclooxygenase2 ， cox - 2 ) 、胶质源性神经营养因子( glialcellline - derivedneutrophicfactor ， gdnf ) 、 caspase - 3基因及蛋白表达的时间性变化，详尽系统地阐述脑损伤后各指标变化的时间规律性及表达差异可能的形成机制。 - The results of the experiments indicates that concentration of aminosilane influences the fluorescence background of glass slide , and some factors affect immobile ratio of oligonucleotide probe , such as aminosilane treatment time , aldehyde treatment time , uv crosslinking energy , washing temperature and time
研究表明，氨基化试剂浓度对玻片荧光背景有影响，氨基化试剂处理时间、醛基化处理时间、紫外交联能量和洗涤温度和时间等工艺因素影响寡核苷酸探针的固定率。
Related Words
- oligonucleotide
- oligonucleotide hybridization
- mutagenic oligonucleotide
- degenerate oligonucleotide
- synthetic oligonucleotide
- oligonucleotide synthesizer
- antisense oligonucleotide
- phosphorothioate oligonucleotide
- sulfurized oligonucleotide
- oligonucleotide microarray
- oligonucleotide micro-chip
- oligonucleotide microarray
- oligonucleotide probe database
- oligonucleotide probe hybridization